Cloprostenol sodium is administered to animals by injection, typically either intramuscularly or intrauterine, depending on the species and purpose of use. It has a rapid onset of action and is highly effective, with ovulation usually occurring within 2-3 days after treatment. In dairy cows, it can be used to shorten the reproductive cycle and improve pregnancy rates, as well as to induce abortion or treat certain reproductive disorders.

While cloprostenol sodium is generally safe for use in animals, some potential side effects may occur, such as transient diarrhea, uterine contractions, or mild discomfort at the injection site. However, these adverse reactions are usually mild and short-lived, and do not pose a significant health risk to the animal.